Select the genome you want to validate sgRNA sequences against.

This one is easier on a computer. It works best with a column of sequences pasted in and a wide table to read the answer from, and it fetches a 36 MB index the first time you use it.

Starting points for a focused screen, not definitive gene families — load one and edit it down. Showing human lists, matching the library selected in step 1. Every symbol has been checked against that species’ built-in libraries, so each list contains only genes at least one library can target.

• Gene-level copy number from DepMap’s OmicsCNGene dataset (24Q4 release).
• Per-gene CN — the values you’ll see in the results table after activating CN mode. Each value is relative to that cell line’s own genome-wide baseline: 1.0 = typical, ≥ 3.0 = amplification, ≤ 0.5 = deletion.
• Per-line ploidy — the small number next to each cell line in the list below (e.g. 2.1n, 3.9n). This is a separate quantity from the per-gene CN above: it describes the line’s overall genome content (average DNA content per cell relative to a haploid genome). 2.0n is diploid. The WGD chip is a separate flag meaning the line’s genome went through a whole-genome doubling event at some point in its history — the line transiently became tetraploid (~4n), but subsequent chromosome loss often brings current ploidy back down, so WGD lines commonly sit anywhere from ~2.5n to ~4n. The results page combines ploidy with the per-gene CN to estimate actual copy counts. If no number is shown, DepMap hasn’t measured ploidy for that line and it’s assumed diploid for the rescaling.
• More cell-line data: depmap.org or the cell line browser in Correlate (Green Listed’s sister app).